Fig. 4

Detailed analysis of bacterial motility. RABiTPy tracks the motility of the gliding bacterium F. johnsoniae as a use case, generating comprehensive quantitative metrics, including (A) overlay of RABiTPy trajectories on images at frames 250, 850, and 1500. B Log–log plot of mean squared displacement (MSD) versus lag time, showing individual cell trajectories (gray lines) and the ensemble MSD (blue line). C Aspect ratio distributions (cell length/cell width) representing the morphology of the cells in the video. D Correlation of speed and area, where individual cells are plotted as blue dots, and the red line shows a negative correlation. E Trajectory and angle analysis of a representative cell, including the simplified trajectory with turn points (top panel, color-coded by time) and angular changes as a function of distance traveled (bottom panel). F Distributions of turn behaviors, with the top panel showing the angle frequency distribution and the bottom panel showing cell-wise turn frequency, where each colored bar represents the turn frequency of a single cell