Fig. 2
From: RABiTPy: an open-source Python software for rapid, AI-powered bacterial tracking and analysis

Usage of traditional thresholding and AI-based methods for segmentation across diverse bacterial species. RABiTPy demonstrates segmentation performance using algorithm-based or adaptive thresholding and AI-based methods on bacterial images with varying shapes and densities. While both approaches enable accurate segmentation, AI-based methods excel in densely populated and crowded environments, ensuring reliable motility tracking