Validating a web application’s use of genetic distance to determine helminth species boundaries and aid in identification

Table 2 Primers used for ABIapp validation with actual specimens

Specimen	Genetic marker	Primer (5′-3′)	Reference
Trichuris sp.	COI	HC02198F: TTTTTTGGGCATCCTGAGGTTTAT	[32]
		CORA: ACYACATAGTAGGTRTCATG
	12S	12S-C1-F: GTGCCAGCTAYCGCGGTTA	[33]
		12S-C1-R: GRTGACGGGCRATATGTG
	16S	16S-C1-F: ACGAGAAGACCCTRGRAAYT	[33]
		16S-C1-R: GRTYTAAACTCAAATCACG
	18S	1096F: GGTAATTCTGGAGCTAATAC	[34]
		1912R: TTTACGGTCAGAACTAGGG
		1813F: CTGCGTGAGAGGTGAAAT
		2646R: GCTACCTTGTTACGACTTTT
Gnathostoma sp., Tanqua sp., and Oesophagostomum stephanostomum	COI	JB3: TTTTTTGGGCATCCTGAGGTTTAT	[35]
		JB4.5: TAAAGAAAGAACATAATGAAAATG
	12S	12S-C345-F: GTWCCAGAATAATCGGMTA	[33]
		12S-C345-R: ATTGAYGGATGRTTTGTRC
	16S	16S-C345-F: AAGATAAGTCTTYGGAARYT	[33]
		16S-C345-R: GAAYTAAACTAATATCAMG
	18S	1096F + 1912R, 1813F + 2646R	[34]
Trematode and cestode	COI	JB3 + JB4.5	[35]
	12S	Tre12S-F: GTGCCAGCADYYGCGGTTA	[37]
		Tre12S-R: AGCAGCAYATHGACCTG
		Ces12SF: GTGCCAGCATCYGCGGTTA	[36]
		Ces12SR: GGTGACGGGCGGTGTGTAC
	16S	CesTre16S-F: GTGYDAAGGTAGSATAAT	[37]
		CesTre16S-R: CCGGTYTYAACTCARCTCAT
	18S	Cfor: ATGGCTCATTAAATCAGCTAT	[38]
		Arev: TGCTTTGAGCACTCAAATTTG

ISSN: 1471-2105