Fig. 6
From: TockyPrep: data preprocessing methods for flow cytometric fluorescent timer analysis
Visualization of timer thresholding using plot_tocky. This figure shows Timer Blue and Red fluorescence of T cells before normalization. T cells from Nr4a3-Tocky::OT-II double transgenic mice were activated by Ova peptide and analyzed at the indicated time points [4]. Quadrant gates illustrate the thresholds for Timer Blue and Red fluorescence set by timer_transform