Fig. 9
From: Marigold: a machine learning-based web app for zebrafish pose tracking
slc1a2btk57/tk57 and wild-type embryos perform rostral–caudal body bends dependent on interactions between genotype and touch location. A Visualization of absolute rostral–caudal angle magnitudes at the peaks of high amplitude body bends, depicted over time according to overall response progress. Three fish were omitted at random in order to represent equal numbers of fish across conditions. Red line indicates the threshold of 110 ° for classification as a high amplitude body bend. B Percentage of responses which contain one or more high amplitude body bends. C Quantification of peak angle of first body bend. D Quantification of time to peak angle of first body bend. E Quantification of maximum angle across the entire response. F Quantification of time to maximum angle across the entire response. Statistical significance determined by two-way ANOVA with Tukey post hoc; n.s.: not significant; ***: p < 0.001; n = 21–24 fish per condition across 3 independent experiments