Table 2 sgRNAs and N20 sequences of each test group
From: GLiDe: a web-based genome-scale CRISPRi sgRNA design tool for prokaryotes
Test group | sgRNA sequence | N20 sequence |
|---|---|---|
R1-NC | TATTATCGGAGCCCGGACAT | TCCTTCCTCTGGTTCCACGG |
R1-EXP | TAATTCGAATGGTTCCACGG | |
R1-PC | TCCTTCCTCTGGTTCCACGG | |
R2-NC | TATTATCGGAGCCCGGACAT | CGGATGGGCCCACCACACCG |
R2-EXP | GCACGATGCCCACCACACCG | |
R2-PC | CGGATGGGCCCACCACACCG | |
R3-NC | TATTATCGGAGCCCGGACAT | GCGCGGCGATGGCTTCGGCG |
R3-EXP | TTCTGCGCATGGCTTCGGCG | |
R3-PC | GCGCGGCGATGGCTTCGGCG | |
R4-NC | TATTATCGGAGCCCGGACAT | TCTCTTGAATCGCTCAACAT |
R4-EXP | GAGCTCAGATCGCTCAACAT | |
R4-PC | TCTCTTGAATCGCTCAACAT | |
NC-blank | TATTATCGGAGCCCGGACAT | CATGTATTATACACGAAGTT |
R1-blank | TAATTCGAATGGTTCCACGG | |
R1-PC-blank | TCCTTCCTCTGGTTCCACGG | |
R2-blank | GCACGATGCCCACCACACCG | |
R2-PC-blank | CGGATGGGCCCACCACACCG | |
R3-blank | TTCTGCGCATGGCTTCGGCG | |
R3-PC-blank | GCGCGGCGATGGCTTCGGCG | |
R4-blank | GAGCTCAGATCGCTCAACAT | |
R4-PC-blank | TCTCTTGAATCGCTCAACAT |