Table 1 An example of four high rank sgRNAs designed by existing tools and their potential off-target hits identified by GLiDe
From: GLiDe: a web-based genome-scale CRISPRi sgRNA design tool for prokaryotes
No | Design tool | Rank | Target gene | sgRNA sequence | Off-target gene | Potential off-target hits discovered byGLiDe |
|---|---|---|---|---|---|---|
R1 | chopchop | 1 | Cgl0025 | TAATTCGAATGGTTCCACGG | Cgl2364 | TCCTTCCTCTGGTTCCACGG |
R2 | chopchop | 3 | Cgl0044 | GCACGATGCCCACCACACCG | Cgl1968 | CGGATGGGCCCACCACACCG |
R3 | Synthego | 3 | Cgl0005 | TTCTGCGCATGGCTTCGGCG | Cgl0369 | GCGCGGCGATGGCTTCGGCG |
R4 | Synthego | 2 | Cgl0059 | GAGCTCAGATCGCTCAACAT | Cgl1680 | TCTCTTGAATCGCTCAACAT |