Fig. 8

Compared with WT TDP-43, TDP-43 mutant A315T induced more DNA damage, as calculated with a one-sided t test. A Representative images of mouse cortical neurons stained with TDP-43 and DAPI. The selection of cells treated with puromycin resulted in many apoptotic nuclei; however, staining for TDP-43 revealed surviving transduced neurons. B The upper panel shows DNA damage areas identified using SimplySmart_v1 in neuronal nuclei expressing A315T (color-mapped regions). The graphs show that the average number of DNA damage foci in the WT TDP-43 group was significantly lower than that in the A315T group (*p < 0.05, one-sided t test), whereas the foci area was unchanged. The images were analysed with SimplySmart_v1 with a threshold value = 140, a marker area range of 1000–5000 pixels, and a minimal size > 2 pixels. Forty-nine nuclei from the WT group and 38 from the A315T group were analysed from 3 technical replicates