Fig. 1
From: PyChelator: a Python-based Colab and web application for metal chelator calculations

Example uses of PyChelator. A An example shows how to calculate the free calcium concentration from the total calcium in the assay buffer used in the in vitro motility assay. B The manual entry of stability constants for other metals and chelators. In this case, constants for Zinc and Calcium buffered using EDTA and EGTA were used [11]