Fig. 4

Demonstration of an in silico analysis using Multioviz on a heterogeneous stock of mice dataset from the Wellcome Trust Centre of Human Genetics. We applied Multioviz to visualize a GRN with associated SNPs and enriched genes driving the architecture of CD8+ cell percentage. To generate this GRN, we set the following parameters in the Multioviz software: (i) Molecular Level 1 (ML1) Map Type = None; (ii) Molecular Level 2 (ML2) Map Type = Complete; (iii) ML1 Threshold = 0.5 which follows the median probability model [19]; (iv) ML2 Threshold = 0.5; and (v) GRN Layout = “layout_with_kk”. In this figure, SNP-level variables in ML1 (red circles) map to gene-level variables in ML2 (blue squares). Upon deleting the  CEL-17_31069801 SNP and rerunning Multioviz, we observe a new association with the SNP CEL-17_31214920 and a new enrichment of several genes, including Anapc1 and Pard3. These are depicted in the perturbed GRN on the right. Note that both the deleted and newly enriched SNPs map to the hlb156 gene