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Fig. 2 | BMC Bioinformatics

Fig. 2

From: A pipeline for RNA-seq based eQTL analysis with automated quality control procedures

Fig. 2

Expr_QC. Identification of outlying samples and normalization of RNA-seq based gene expression data. a Gene expression levels of two gender-specific genes: RPS4Y1 and XIST. b The RLE plot before data preprocessing. Aligned box-plots, in increasing order of IQR, represent residuals of gene expression for samples. c Hierarchical clustering of samples before data preprocessing. Color represents the batch of library preparation. d Distribution of D-statistics before data preprocessing. e Hierarchical clustering dendrogram after adjusting batch effects. f The RLE plot after data preprocessing. g Hierarchical clustering dendrogram after data preprocessing

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