Fig. 1

Flow chart of the tools and steps used in the automated workflow carried out by AIDD pipeline. The analysis begins from gathering relevant RNA-seq data files from the NCBI SRA database, followed by reads alignment using HISAT2 with Ensembl annotations. Transcriptome assembly is then performed by Stringtie. Downstream expression analysis can be performed using multiple tools, including DESeq2, edgeR and topGO. Variant calling to detect RNA-editing events, including A-to-I editing, is performed using tools implemented in GATK; and statistical analysis of the effect of RNA editing is performed using custom R scripts